Microsatellite instability of 10 human cancer cell lines

doi:10.3969/j.issn.2095-4344.2015.07.013

Abstract

Abstract:

BACKGROUND: We aim to understand microsatellite instability in various human cancer cells.

OBJECTIVE: To analyze the difference in genotypic background of various tissue-derived human cancer cells based on microsatellite instability.

METHODS: Ten human cancer cell lines at exponential phase were cultured, including human leukemia cells, colon cancer cells, stomach cancer cells, liver cancer cells, lung cancer cells, cervical cancer cells, bladder cancer cells, and two normal human cell lines (human umbilical vein endothelial cells and human skin fibroblasts), and their microsatellite stability statuses were detected by five quasi-monomorphic markers: BAT-25, BAT-26, CAT-25, SEC-63, NR-24. DNA of 10 human cancer cell lines, human umbilical vein endothelial cells and human dermal fibroblasts was extracted and amplified by PCR. PCR products were detected by 12% polyacrylamide gel electrophoresis (PAGE)-silver staining and sequenced by ABI 3720XL automatic sequencer.

RESULTS AND CONCLUSION: For all the five microsatellite markers detected, the length of their PCR amplified fragments was nearly identical in human umbilical vein endothelial cells and human dermal fibroblasts, demonstrating their quasi-monomorphic nature. When compared with human umbilical vein endothelial cells and human dermal fibroblasts, amplified fragments in human colon cancer cells (HCT-116) and human bladder cancer cells (BIU-87) were shortened for 7-13 bp at BAT-25, BAT-26, CAT-25 or NR-24, respectively. No obvious differences were found at all the five loci in other cells. According to the results, HCT-116 cells and BIU-87 were considered as cells with high-level microsatellite instability. Other cell lines exhibited microsatellite stability at five loci, and they were considered as microsatellite stable cells. Our study revealed status of genome instability in 10 human cancer cell lines, providing references for appropriate cell line selecting in future drug screening and basic medical research.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Carcinoma, Cell Line, Tumor, Microsatellite Instability

CLC Number:

R318

Shi Zhong-zheng, Liu Yan, Luo Min, Chen Ying-jie, Zhou Yi-ping. Microsatellite instability of 10 human cancer cell lines[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(7): 1052-1056.

Figures/Tables 2

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